超声辅助溶剂棒微萃取-液相色谱法快速测定婴儿配方奶粉中的双酚A

摘要

A technique for the rapid determination of bisphenol-A (BPA) in infant formula milk powder was developed by ultrasound-assisted solvent bar microextraction coupled with high performance liquid chromatography.The system of ultrasound -assisted solvent bar microextraction was designed.The optimized microextraction conditions were as follows:the length of solvent bar was 30 mm,1-octanol was used as the extraction solvent,the stirring rate was 600 r/min,the ultrasonic time was 10 min and the extraction time was 15 rain,the pH of sample solutions was 6,0.12 mol/L of NaOH was used as the acceptor phase,the concentration of NaCl was 60 g/L Under the optimal microextraction conditions,the average enrichment factor was 102,the linear range was 0.1 ～ 100 μg/L (r =0.9989),the limits of detection was 0.35 μg/L (S/N =3),and the relative standard deviations(RSD)was lower than 4.32％ (n =6).The proposed method was applied to the determination of BPA in infant formula milk powder samples.The spiked recoveries were in the range of 89.7％ ～102.8％.It was showed that the proposed method was environmentally for the rapid determination of BPA in infant formula milk powder.%建立超声辅助溶剂棒微萃取-高效液相色谱(UA-SBME-HPLC)方法,用于快速测定婴儿配方奶粉中的双酚A(BPA).设计超声辅助溶剂棒微萃取系统,优化后的最佳萃取条件为:溶剂棒的有效长度为30 mm,萃取溶剂为正辛醇,搅拌速度为600 r/min,超声时间10 min,萃取时间15 min,pH =6的HCl溶液为给体相,0.12 mol/L NaOH溶液为受体相,NaCl的加入浓度为60 g/L.在最优萃取条件下,该方法对BPA的平均富集倍数为102倍,方法的线性范围为0.1～ 100 μg/L(r =0.9989),检出限为0.35μg/L(S/N=3),相对标准偏差RSD小于4.32％(n=6),加标回收率为89.7％～102.8％.研究表明该方法绿色环保,可用于准确快速测定婴儿配方奶粉中的双酚A.