真菌毒素DON和ZEN的体外联合毒性评价

摘要

BEL-7402 cells were chosen as the cell model in this study to evaluate the indiviual and combined toxicity of Deoxynivalenol (DON)and Zearalenone (ZEN).With the logarithmic phase of BEL7402 cells exposed to different concentrations of DON,ZEN and their mixture for 24 h,the rate of cell proliferation,reactive oxygen species (ROS) and calcium levels,cell apoptosis rate and apoptosis related genes expression were analyzed and the combined effect of the two toxins was evaluated.The results showed that the mycotoxin DON,ZEN could inhibit cell activity with an IC50 of 9.3 μg/mL and 27.2 μg/mL,lead to cell damage induced by oxidative stress and increase the intracellular levels of reactive oxygen species and calcium ion concentration.Proapoptotic gene p53 was signifigantly up-regulation and Bal-2 gene was down-regulated,induce cell apoptosis or necrosis,a variety of toxic index verified that the combined toxicity of these two toxins on cells have an additive effect.%选取BEL7402细胞对脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)和玉米赤霉烯酮(Zearalenone,ZEN)的联合毒性进行评价.首先将不同浓度的DON、ZEN以及其混合物染毒对数期的BEL7402细胞,刺激24h后,对细胞增殖率、ROS活性氧水平、钙离子水平、细胞凋亡率以及凋亡相关靶点基因的表达等指标进行监测分析,并对两种毒素的联合毒性作用进行评价.结果表明,真菌毒素DON、ZEN均可抑制细胞活性,IC50分别为9.3、27.2 μg/mL,可导致细胞氧化应激损伤,提高细胞内活性氧水平以及钙离子浓度,显著上调凋亡关键基因p53、Bax,下调Bal-2基因,诱导细胞产生早期凋亡甚至坏死,多种毒性指标验证了这两种毒素对细胞的联合毒性作用表现为加和作用.