低分子量胶原蛋白肽交叉苦瓜素辅助降低糖尿病小鼠血糖作用特性

摘要

The hypoglycemic effects of low molecular weight collagen peptide intersecting Momordica charantia extracts were determined in mice diabetic model in vivo.The diabetic model mice were administrated collagen peptide and Momordia charantia extracts through intragastric administration and then the value of fasting glucose,body weight,organ mass and liver antioxidant capacity of the mice and given oral glucose tolerance test were measured.After the treatment of collagen peptide intersecting Momordica charantia extracts,the blood sugar value was decreased to 7.5～16.63 mmo]/L,liver index and kidney index decreased to 44.98～56.17 mg/g,11.29～17.95 mg/g respectively.The spleen index increased to 2.71 ～3.52 mg/g,Area Under Curve reduced to 32.03～38.14 mmol/(min·L).Superoxide dismutase activity increased to 89.18～154.72 U/mg protein,and the content of MDA decreased to 1.66～3.00 nmol/mg protein.Compared with the model group,the bone collagen peptide of Epinephelussp intersecting Momordica charantia extracts of the middle dosage treatment group,and the skin collagen peptide of Oreochromis mossambicu intersecting Momordica charantia extracts of middle and high dosage treatment groups effect were remarkable.The blood sugar value at 28 days and AUC at 21 days decreased significantly,liver index and kidney index decreased,but the spleen index increased.The antioxidant ability of liver tissue increased.The results showed that the treatment of collagen peptide intersecting Momordica charantia extracts could significantly reduce the blood glucose value(p ＜ 0.05),and the blood glucose value related to the improvement of liver antioxidant capacity of diabetic mice model.Meanwhile,the treatment of collagen peptide intersecting Momordica charantia extracts could promote organ repair and improve the ability of autoimmunity.%采用小鼠糖尿病模型研究低分子量胶原蛋白肽交叉苦瓜素辅助降血糖的作用特性.对糖尿病模型小鼠进行胶原蛋白肽交叉苦瓜素灌胃处理,测定给药前后小鼠的空腹血糖值、体重、脏器指数与肝组织抗氧化能力,进行糖耐量实验.结果表明,胶原蛋白肽交叉苦瓜素将糖尿病模型小鼠的血糖含量降低至7.5～17.8 mmol/L,葡萄糖耐量曲线下面积(AUC)降低至32.62～38.66 mmol/(min·L),肝脏指数和肾脏指数降低至44.98～57.17 mg/g和11.29～17.95 mg/g,脾脏指数提高至2.71～3.52 mg/g,超氧化物歧化酶(SOD)活力增强至88.18～158.38 U/mg prot,丙二醛(MDA)含量降至1.66～3.00 nmol/mg prot.与模型组相比,胶原蛋白肽交叉苦瓜素处理的糖尿病模型小鼠,第28 d的血糖值和第21 d的AUC显著降低(p＜0.05),肝脏指数和肾脏指数下降,脾脏指数上升,肝组织抗氧化能力提高,其中石斑鱼骨胶原蛋白肽交叉苦瓜素的中剂量组与罗非鱼皮胶原蛋白肽交叉苦瓜素的中剂量组和高剂量组效果较显著.研究表明胶原蛋白肽交叉苦瓜素可使糖尿病小鼠的血糖值显著降低(p＜0.05),且血糖值降低与肝脏抗氧化能力提高相关联,同时胶原蛋白肽交叉苦瓜素可促进糖尿病模型小鼠的脏器损伤修复,提高其自身免疫能力.