墨西哥黄唇鱼鱼肉胶原肽促进小鼠伤口愈合作用

摘要

以人工增殖放流的墨西哥黄唇鱼肉为原料,经复合酶解得到平均分子量为837 Da的寡肽(Totoaba macdonaldi-meat collagen peptides,TMCP),口服给药探索其对皮肤创伤模型小鼠伤口愈合的促进作用.取40只体重为(25±2)g的健康雄性ICR小鼠,随机分为4组,每组10只,在其背部制造直径为8.0 mm的皮肤切除型伤口.造模后次日起,分别用低、中、高三个剂量(0.66、1.33和2.0 g/kg·bw)的TMCP和等体积的去离子水(空白对照)连续灌胃14 d.每隔2d测定伤口愈合率,分别于造模后的第7和第14 d分批处死动物,取伤口边缘皮肤组织,用于HE染色和Masson染色,并进行羟脯氨酸含量测定和免疫组化分析.结果表明,在第7、14 d时,TMCP三个处理组均优于空白对照组,且中、高剂量组与之均呈现极显著差异(p＜0.01);第14 d时,TMCP低剂量组也在伤口愈合率、胶原含量、血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)及碱性成纤维细胞生长因子(Basic Fibroblast Growth Factor,bFGF)表达量指标上呈现极显著差异(p＜0.01).研究表明,TMCP有效促进了小鼠伤口的胶原表达和组织重塑,有效促进创伤性动物皮肤伤口的愈合,具有作为口服营养保健品开发的潜力,为取鳔后墨西哥黄唇鱼的高值转化提供了思路.%Totoaba macdonaldi-meat collagen peptides (TMCP)with molecular weight of 837 Da obtained from Mexican farmed Totoaba used combinative enzyme to investigate its effects on wound healing in mice.40 healthy male ICR mice were underwent surgical full-thickness incision with a diameter of 8 mm and were divided into 4 groups,which were normal control group and 0.66,1.33,2.0 g/kg.bw TMCP groups.TMCP were orally administered by gavage to mice in TMCP group from post-operation,and the control mice were given vehicle only for 14 d.The healing of the wound were observed every 2 d.Necropsy was performed on postoperative 7 and 14 d.The wound tissues were excised for HE staining,Masson staining,hydroxyproline measurement and immunohistochemical analysis.According to results,TMCP-M and TMCP-H had highly significant positive effect on all indexes (p ＜ 0.01).TMCP-L could highly significantly increase the healing rate,collagen content,VEGF and bFGF expression on 14 d(p ＜ 0.01).TMCP groups could promote collagen producing and tissue remodeling.TMCP had potential to promote wound healing and could be used in high-quality oral healthy products,that would provide a theoretical basis for high -efficient utilization.