毛细管电泳电化学发光法快速分离检测水果中的精胺

摘要

目的:建立水果中精胺含量测定的毛细管电泳电化学发光(CE-ECL)法.方法:利用CE-ECL技术建立了一种高效快速准确的测定桑椹、苹果、草莓中精胺含量的的方法.结果:精胺标准溶液在2×10-4～3×10-1 mmol/L浓度范围内与ECL强度呈良好的线性关系,其线性方程为I=1114.2c +99.41,相关系数r=0.9996(n =5),检出限(s/N=3)为9.9×10-5 mmol/L,加标回收率分别为95.14％ ～101.15％、98.97％ ～ 100.82％和100.57％～106.11％.结论:该方法简单、准确,重复性好,可用于研究果蔬采后保鲜贮藏和延长货架期.%Objective:To establish a capillary electrophoresis with electrochemiluminescence (CE-ECL)method for determination of spermidine in fruit.Methods:An efficient,rapid and accurate analytical approach was developed for the determination of spermine in mulberry,apple,strawberry by CE-ECL technique.Results:The ECL intensity was linear with the concentrations of spermine over the ranges from 2 × 10-4 ～3 x 10-1 mmol/L.The linear equation was I =1114.2c + 99.41,correlation coefficient r =0.9996 (n =5) with detection limit (S/N =3) of 9.9 × 10-5 mmol/L.The adding standard recoveries were 95.14％ ～ 101.15％,98.97％ ～ 100.82％ and 100.57％ ～ 106.11％,respectively.Conclusions:This method is simple,accurate,reproducible and can be used for the study of fruit fresh-keeping,and prolonging processing period.