升阳十一味丸对胃癌细胞凋亡及细胞周期的影响

摘要

To studay the effect of Inner Mongolia Drug Shengyangshiweiwan on cell apoptosis and cell cycle in humand gastric cancer MGC-803 cell, MTT assay was used to examine the proliferation inhibitory effect of shengy-angshiyiweiwan on MGC-803 cell, and calculate the inhibitory rate of growth. Morphologic changes of apoptosis were observed by reverse discrepancy and offluorescent light microscope; flow cytometry was used to analysis cell cycle. MTT assay showed that shengyang has proliferation inhibiting effect on MGC-803 cell in dose and time de-pendent manners. The cells treated with shengyang (2.5 mg/mL) for 48 hours showed characters of apoptosis un-der light microscope. An ' sub-G1 peak was detected by flow cytometry, the result analysis of cell cycle indicated that the number of cells of S phase increased, that of G0/ G1 and G2/M phase decreased after treatment on MGC-803, it was arrested S phase. It is conclused that shengyang has proliferation inhibiting effect and may induce apop-tosis in gastric cancer MGC-803 cell in a certain dose range, which may be one of the antitumor mechansim.%研究复方蒙药升阳十一味丸对体外培养的人胃癌MCC-803细胞凋亡及细胞周期的影响.以不同浓度的蒙药处理MGC-803细胞,通过MTT法检测药物作用后细胞的增殖情况,并计算生长抑制率.利用倒置光学显微镜、Hochest33342/PI荧光染色、流式细胞术,研究蒙药诱导细胞凋亡的作用及对细胞周期的影响.结果蒙药能抑制MGC-803细胞的增殖,并具有良好的量效关系.2.5 mg/mL药物作用MGC-803细胞48 h后,光镜下可见核固缩、核碎裂等凋亡细胞形态学变化.流式细胞仪检测到“亚G1峰”,细胞周期分析表明S期细胞较对照组增多,G2/M期细胞下降,阻滞于S期.说明蒙药升阳可明显抑制胃癌MGC-803细胞的增殖,并在一定浓度范围内诱导细胞凋亡,这可能是其发挥抗肿瘤的作用主要途径之一.