金属离子及表面活性剂对纤维素酶水解预处理玉米秸秆的影响

摘要

Influences of metal ions and surfactants on the hydrolysis of pretreated corn stover by cellulase were researched. It was showed that some metal ions and surfactants loading in hydrolysis system, such as Cu2+ and Tween80, could enhance the activity of cellulase. In a 50 g hydrolysis system at pH 5.0, temperature 50 degrees centigrade , pretreated corn stover ( PCS) 10 wt％ , enzyme loading 1 g ( 1300 from Zensun Sci & Tech Co. , Ltd) ,hydrolysis time 3 hours, the reducing sugar concentration with Tween80 (20 mg/L) loading is 21.3 percent of no Tween80 loading, and reducing sugar concentration with Cu2+ (0.13 mmol/L) loading is 138. 2 percent of no Cu2+loading.%研究了不同的金属离子及表面活性剂对纤维素酶水解的影响,试验表明添加某些金属离子及表面活性剂能够促进纤维素酶的水解,其中Cu2+和Tween80促进效果最佳.50g反应体系在pH 5.0、温度50℃、预处理玉米秸秆(PCS)10 wt%、1300纤维素酶(泽生科技)加入量1g条件水解3 h,添加20 mg/L Tween80的水解液中还原糖浓度比不加Tween80时提高21.3%;添加0.13 mmoL/LCu2+的水解液中还原糖浓度比不加金属离子时提高38.2%.