Biological denitrification is considered to be the best option for removing nitrate from wastewater. The purpose of this study was to solve the problem of the lack of carbon source for biological denitrification in water re-mediation. Methanol, ethanol and glucose were selected as carbon sources to evaluate their capacity for the promo-tion of denitrification. And then, the effect of C/N ratio and temperature on denitrification was investigated. The results showed that methanol, ethanol and glucose can be used as carbon source for biological denitrification and ethanol showed the best effect for the promotion of denitrification owing to the fastest denitrification rate, higher denitrification efficiency and lower accumulation of nitrite and ammonia nitrogen. The C/N ratio had significant effect on denitrification process, the higher C/N ratio, the faster nitrate removal rate. In addition, the temperature has an important influence on denitrification, and the nitrate removal efficiency at 25 ℃, 35 ℃ were much better than that at 10 ℃.%生物反硝化法是去除水体中硝酸盐的有效方法。鉴于生物反硝化过程中有机碳源不足的问题,选择甲醇、乙醇、葡萄糖作为反硝化碳源,研究它们对反硝化的促进作用；同时研究C/N比以及温度对反硝化过程的影响。结果显示：甲醇、乙醇和葡萄糖作为反硝化碳源时,均可获得较高的硝酸盐氮去除率。以乙醇为碳源时,反硝化速率进行的最快,硝酸盐氮去除率高,中间副产物亚硝酸盐氮和氨氮积累少,是最优的反硝化碳源；C/N比对反硝化过程影响显著,C/N比越高,脱氮速率越快；另外温度对反硝化也有着重要的影响,在25℃、35℃时的脱氮效果远好于10℃时的脱氮效果。
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