基于新型乙酰胆碱酯酶传感器的有机磷农药检测

摘要

Hollow carbon spheres - ionic liquid 1 - butyl -3 - methyl imidazole phosphotungstate (HCS -[Bmim]3 PW12 O40 )was prepared to modify glassy carbon (GC)electrode.Acetylcholinesterase (AChE)was immobilized on the electrode surface by cross -linking reaction to obtain AChE /HCS -[Bmim]3 PW12 O40 /GC sensor.Under the optimal condition,the organophosphorus pesticides,monocro-tophos and carbofuran,were detected by the biosensor.The linear range of monocrotophos was 8.00 × 10 -10 ~8.00 ×10 -6 g/L,and that of carbofuran 1.00 ×10 -10 ~1.00 ×10 -7 g/L,with the detection limits of 7.58 ×10 -11 g/L and 6.76 ×10 -11 g/L,respectively,calculated by inhibition rate 10%.The biosensor exhibited high sensitivity and acceptable stability,and could be a promising tool for analysis of organophosphorus pesticides.%制备了空心碳球—离子液体1－丁基－3－甲基咪唑磷钨酸盐（HCS －[Bmim]3 PW12 O40）修饰玻碳（GC）电极，采用交联法将乙酰胆碱酯酶（AChE）固定在修饰电极表面，获得 AChE ／HCS －[Bmim]3 PW12 O40／GC 传感器。在最优条件下，用该传感器对有机磷农药久效磷、克百威标准品分别进行检测，久效磷的线性范围为8．00×10－10～8．00×10－6 g／L，克百威的线性范围是1．00×10－10～1．00×10－7 g／L。按抑制率10％计算，久效磷和克百威的检出限分别为7．58×10－11 g／L 和6．76×10－11 g／L。所制备的 AChE ／HCS －[Bmim]3 PW12 O40／GC 传感器灵敏度高和稳定性好，为有机磷农药的分析提供了一种有前景的工具。