免疫亲和柱净化HPLC柱后光化学衍生法测定34批中药材中黄曲霉毒素G2、G1、B2、B1

摘要

目的 建立HPLC柱后光化学衍生法检测中药材中黄曲霉毒素G2、G1、B2、B1方法.方法 样品经过70％甲醇提取、免疫亲和拄净化后,采用HPLC柱后光化学衍生-荧光检测器检测中药材黄曲霉毒素含量.对疑似成分进行液质确认.结果 黄曲霉毒素G2和B2,G1和B1分别在0.75 ～ 22.5 pg和5～ 75Pg线性关系良好,方法准确稳定.检测的34批次药材中,3批酸枣仁检出黄曲霉毒素,其中1批酸枣仁黄曲霉毒素B1超过5μg·kg-1.结论 该方法简便、准确,适用于中药材黄曲霉毒素的检测.%Objective To establish HPLC methods associated with post column Photochemical Derivatization to determine aflatoxin G2, Gl, B2, Bl in Chinese herbal. Method Aflatoxins were extracted by 70% methanol and purified by an immunoaffinity column. Then the samples were analysed by HPLC fluorescence detector with post column Photochemical derivatization. Confirm the suspected components with LC-MS. Results The method with the great linear concentration range of 0.75-22.5 pg for aflatoxins G2,B2,and 5-75 pg for aflatoxins G1,B1 respectively, was stable and accurate. In the result of 34 batches of Chinese herbs,Aflatoxins were detected in 3 batches of Semen Ziziphi Spinosae among which Aflatoxin Bl of one batch exceed 5 μg·kg-l. Conclusion The method is simple and accurate,which is suitable for the determination of aflatoxins in Chinese herbal.