Slug 在口腔鳞癌中的表达及在上皮间质化中的作用

摘要

Objective To investigate the expression of Slug in oral squamous cell carcinoma and its influence in the process of epithelial mesenchymal transitions.Methods The expression of Slug, E-cad,Vimentin in oral squamous cell carcinoma was detected by SP immunohistochemistry and in situ hybridization method in 42 specimens of oral squamous cell carcinoma and 12 specimens of oral normaltissue.Results The positive rate of Slug expression in oral squamous cell carcinoma was significantly higher than that in normal tissue(P <0.01);The expression of Slug related to lymph metastasis and pathological grading(P <0.05),but not related to the age of patients(P >0.05);The correlation between Slug and E-cad was negative,but was not between Slug and Vi-mentin.Conclusion Slug expression might play an important role in the development,metastsis of oral squamous cell carcinoma;EMT might be induced by Slug significantly.%目的：探讨 Slug 在口腔鳞癌中的表达及在上皮间质化中的作用。方法采用免疫组织化学SP 法和原位杂交法检测42例口腔鳞癌及12例正常口腔组织中 Slug、E-cad、Vimentin 的表达，对比 Slug 在两种组织中表达的差异，分析 Slug 与 E-cad 和 Vimentin 之间的关系，探讨 Slug 的表达与口腔鳞癌临床分期和病理分级的关系及其在上皮间质化中的作用。结果Slug 在口腔鳞癌中的阳性表达率明显高于正常组织，差异具有统计学意义(P <0.01)；Slug 在口腔鳞癌组织中的表达与淋巴结转移、肿瘤病理分级有关(P <0.05)，与口腔鳞癌发病年龄无关(P >0.05)；Slug 与 E-cad表达负相关，与 Vimentin 表达正相关。结论Slug 在口腔鳞癌的发生、分化及转移中起重要作用，可能在 EMT 的发生中起诱导作用。