安徽两个鸡场 ALV-J 分离株 gp85基因的克隆及序列分析

摘要

In order to further understand the genetic variation of subgroup J avian leukosis virus (ALV-J)in Anhui, ALV-J strains were isolated,and the gp85 gene was cloned by PCR.And we compared the amino acid sequences with thirteen sequences of ALV-J gp85 genes in the GenBank database.The results showed that ALV-J gp85 gene was highly variable,mutation sites accounted for 22.8% of full length amino acid sequence.hr1and hr2 domains had high mutation frequency,while vr2 and vr3 domains were relatively more conservative.Protein secondary structure prediction showed ALV J gp85 contained much random coil conformation,expecially hr2 domain.The homology between ALV-J gp85 amino acid sequences of strains AH1103 and AH1104 was 95.8%.The highest homology with strain AH1103 were strains BJ090201J and ev/J,and strain AH1104 had the highest homology with strain HuB09JY04 isolated in China.Moreover,evolution analysis further revealed that the genetic distance strains AH1103 and AH1104 with strains NX0101,SD07LK1 and 4817 was farther.%为研究 J-亚群禽白血病病毒（ALV-J）在安徽省鸡群中的遗传变异趋势，对分离到的2株安徽省ALV-J 毒株，克隆了其 gp85基因序列，并与国内外分离毒株的相应基因序列进行了比较。结果显示，ALV-J gp85基因序列具有高度的易变性，可变氨基酸残基占全长序列的22．8％。其中高变区 hr1和 hr2具有较多的可变位点，而可变区 vr2和 vr3相对较为保守。二级结构预测显示 ALV-J gp85蛋白含有大量的无规则卷曲结构，尤其是 hr2区域。同源性分析表明，毒株 AH1103和 AH1104的 gp85氨基酸序列同源性为95．8％；毒株 AH1103与毒株 BJ090201J，ev/J 的 gp85序列同源性最高，毒株 AH1104的 gp85序列则与国内毒株 HuB09JY04的同源性最高。进化分析显示，毒株 AH1103和 AH1104均与国内毒株 NX0101和SD07LK1及美国分离株4817的遗传距离较远。