北京地区奶牛隐性乳房炎金黄色葡萄球菌的分离鉴定及药敏试验

摘要

为了解北京地区奶牛隐性乳房炎中金黄色葡萄球菌的感染情况及对常用药物的敏感性，用科玛嘉显色培养基和16 S rRNA PCR 检测方法对5个奶牛场的100份隐性乳房炎奶样进行金黄色葡萄球菌的分离鉴定，采用 K-B 纸片扩增法进行药敏试验。结果表明，显色培养基分离到24株疑似金黄色葡萄球菌，经16 S rRNA PCR 鉴定，15株为金黄色葡萄球菌；药敏试验结果显示，15株金黄色葡萄球菌均对β-内酰胺类中的氨苄西林产生普遍耐药性，对克林霉素和环丙沙星的耐药率较高，分别为53．33％和40％；对氧氟沙星、庆大霉素、头孢噻肟和头孢曲松的耐药性较低，耐药率为6．67％。说明15株金黄色葡萄球菌分离株对7类9种抗菌药物都有不同程度的耐药性。%In order to understand the incidence of dairy cow mastitis caused by Staphylococcus aureus and drug susceptibility in Beijing,the Staphylococcus aureus were isolated and identified from 100 samples of five dairy farms with Staphylococcus aureus color culture medium and 16 S rRNA PCR-amplification,and drug sensitivities were tested with K-B disk diffusion method.The results showed that 24 suspected Staph-ylococcus aureus were isolated with color culture medium,15 of which were identified as Staphylococcus aureus by 16 S rRNA PCR-amplification.The results of drug susceptibility showed that 15 isolates of Staphylococcus aureus had complete resistance to ampicillin of beta-lactam antibiotics,the higher resistance rates to clindamycin and ciprofloxacin were 53.33% and 40%,respectively,and the lower resistance rates to ofloxacin,gentamicin,cefotaxime and ceftriaxone were 6.67%.The results showed that 15 isolates of Staphylococcus aureus had different levels of resistance to nine kinds of antimicrobials in seven categories.