基因A型和C型鸭甲型肝炎病毒卵黄抗体的研制及应用

摘要

旨在研制基因 A 型和 C 型鸭甲型肝炎病毒（DHAV）单价及二价高免卵黄抗体，以满足实际生产中基因 A 型和 C 型 DHAV 单独或混合感染防控的需要。采用基因 A 型和 C 型 DHAV 强毒株作为抗原制备单价和二价油乳剂灭活疫苗免疫接种高产蛋鸡，制备了高效价的基因 A 型和 C 型 DHAV 单价及二价高免卵黄抗体，卵黄抗体鸭胚中和效价在1∶204与1∶281之间，单价及二价卵黄抗体防治试验证明，所制备的卵黄抗体防治效果良好。%The objective of this study was to develop monovalent and bivalent egg yolk antibodies against duck hepatitis A virus genotypes A and C to meet the requirement of prevention and treatment of duck viral hepatitis caused by duck hepatitis A virus genotypes A and C.Firstly,virulent duck hepatitis A virus geno-type A and C strains were used as antigens to prepare monovalent and bivalent duck hepatitis A virus geno-type A and C inactivated vaccine in oil-emulsion with Freud's adjuvant.Then the vaccines were used to im-munize layer hens to produce immunized eggs for preparing highly immunized egg yolk antibodies.The good experimental prevention and treatment effects were acquired with both monovalent and bivalent egg yolk antibodies.