2型糖尿病合并肾性高血压模型的建立及评价

摘要

The rat model was induced to simulate the pathogenic process of type 2 diabetes mellitus(T2DM) complicated renal hypertension of human.The type 2 diabetic model of rats was prepared by the quick in-traperitoneal injection of STZ at 28 mg/kg after high calorie and high sugar diet for 4 weeks.The type 2 di-abetic model was established by the measurement FBG≥7.8 mmol/L or RBG≥16.7 mmol/L for succes-sive 3 days after 2 weeks.After the blood sugar reached a steady value,the modeled of renal hypertention was prepared by using the improved method oftow kidney one clipto produce the renal artery stenosis of one side.The model of type 2 diabetes mellitus(T2DM)complicated renal hypertension was successfully replicated by the 3 days′measurement,which showed that the rats′blood pressure SBP≥ 140 mmhg and FBG≥7.8 mmol/L or RBG≥ 16.7 mmol/L two weeks latter.After 4 weeks,the blood sugar and blood pressure of the model rats reached a steady level.Compared with the blank control group,the body weight of the complex model group were decreased,and the fasting glucose,GHbA1c and blood pressure signifi-cantly rosed (P <0.01).Compared with the diabetes group and the sham group,serum creatinine and urea nitrogen of the complex group showed no significant changes(P >0.05),but a significant increase occurred in blood pressure occurred(P <0.01).The result of color ultrasonography showed a shrink of the left kid-ney and renal artery and a faster blood flow velocity.The model of type 2 diabetes mellitus (T2DM)com-plicated renal hypertension established by injecting low-dose STZ intraperitoneally after high fat and high glucose feed and by applying the improved method of tow kidney one clip can simulate the pathogenic characteristics of disease and provide a stable,practical and effective model for the clinic and experiment re-searches of type 2 diabetes mellitus(T2DM)complicated renal hypertension.%制备一种发病过程类似于人类2型糖尿病合并肾性高血压疾病的大鼠模型。大鼠高脂高糖膳食4周后，予快速腹腔注射链脲佐菌素（STZ）溶液28 mg／kg，制作成2型糖尿病模型。2周后，连续3 d 均测得空腹血糖（FBG）≥7．8 mmoL／L 或随机血糖（RBG）≥16．7 mmol／L，为2型糖尿病成模标准。待血糖稳定后，再用改良的“两肾一夹法”结扎肾动脉，造成一侧肾动脉狭窄，形成肾性高血压。2周后，连续3 d 均测得大鼠收缩压（SBP）≥140 mmHg，同时 FBG≥7．8 mmol／L 或 RBG≥16．7 mmol／L，确定为2型糖尿病合并肾性高血压模型制作成功。4周后，模型大鼠血糖、血压稳定。与空白对照组相比，复合模型组大鼠出现体重减轻、空腹血糖升高、糖化血红蛋白值升高（P ＜0．01），血压升高（P ＜0．01）；与糖尿病组和假手术组相比，复合模型组大鼠血肌酐，尿素氮变化不明显（P ＞0．05），但血压明显升高（P ＜0．01）；同时，彩超结果显示，复合模型组大鼠左侧肾脏、肾动脉直径均变小，血流速增快。大鼠高脂高糖膳食后，用低剂量链脲佐菌素（STZ）溶液腹腔注射，再用改良的“二肾一夹”法制备的2型糖尿病合并肾性高血压大鼠模型，在一定程度上较好地模拟出该疾病的发病特点，可为2型糖尿病合并肾性高血压后的临床及试验研究提供一个稳定、实用、有效的新模型。