细胞谱系示踪技术

摘要

Cell lineage tracing is to track certain cell and all of its progeny.From the 20th century, line-age tracing has provided a predominant method to study organ development, tissue repairiaed cell fate-de-termination.Recently, the rapid development of technique for gene engineering, especially the applica-tion of Cre/loxp recombinase system expands the application range of lineage tracing.Here we discuss the application of lineage tracing technique in different studies, and summarize the characteristics and re-cent progresses of this technique.%细胞谱系示踪（ cell lineage tracing）是指利用各种方式标记细胞，并对包括其后代所有细胞的增殖、分化以及迁移等活动进行追踪观察。自20世纪以来，谱系示踪技术为研究器官发育、组织损伤修复以及单细胞的分化命运提供了重要的手段。近些年，随着基因工程技术的飞速发展，细胞谱系示踪技术也有所突破，尤其是诱导性重组酶Cre/loxp系统的应用，极大地拓宽了细胞谱系示踪技术的应用范围。本文将结合细胞谱系示踪技术在多种研究中的应用，对该技术的原理、特点以及最新进展做一综述。