目的:探讨电针预处理对创伤后应激障碍大鼠(PTSD)焦虑样行为的改善作用及对海马去乙酰化酶Sirt1及单胺氧化酶MAO-A基因表达的影响.方法:将32只SD大鼠随机分为对照组(Sham),模型组(PTSD),电针刺激组(EA),电针预处理+模型(EA+ PTSD),每组8只.电针刺激组(EA)和电针预处理+模型(EA+ PTSD)组大鼠每天给予百会穴电针刺激(2/15 Hz,1mA)30min,连续刺激7d.然后对模型组(PTSD)组和电针预处理+模型(EA+ PTSD)组大鼠进行ESPS造模处理.造模结束14天后,通过旷场和高架十字测试其焦虑样行为,随后处死大鼠,通过实时定量PCR(RT-PCR)检测海马Sirt1和MAO-A的mRNA表达情况.结果:(1)PTSD大鼠具有明显的焦虑样行为,包括旷场中心的探索减少(44.94± 13.66,16.86±7.12,P＜0.05)和高架十字开臂运动时间减少(55.90± 26.39,23.36±12.23,P＜0.01),PTSD组大鼠海马Sirt1和MAO-A的mRNA表达增加(1.00± 0.07,1.90±0.05;1.01± 0.10,1.54±0.15,P＜0.01).(2)电针预处理可以缓解PTSD大鼠的焦虑样行为,PTSD与EA+ PTSD组之间存在显著性差异(16.86± 7.12,36.24± 13.28,P＜0.05;23.36± 12.23,50.14± 20.77,P＜0.05).(3)电针预处理可以抑制PTSD大鼠海马的Sirt1和MAO-A mRNA表达,PTSD与EA+ PTSD组之间的Sirt1和MAO-A的mRNA水平存在显著性差异(1.90± 0.05,1.30±0.08,P＜0.05;1.54± 0.15,1.23±0.15,P＜0.05).结论:电针预处理可以调节PTSD大鼠海马的Sirt1和MAO-A的mRNA水平,缓解PTSD大鼠的焦虑样行为.%Objective:To investigate the effect of Electroacuptmcture (EA) preconditioning on anxiety-like behavior and the effect on hippocampus Sirt1/MAO-A expression of PTSD rats.Methods:SD rats were randomly assigned to the following 4 groups (n=8 for each group):Sham,PTSD,EA,EA + PTSD.The EA and EA + PTSD group received EA treatment at frequency of 2/15 Hz for 30 min daily for 7 days.Then,the PTSD and EA + PTSD group rats received SPS&S.After 14 days of treatment with SPS&S,rats were experienced open field test and elevated plus maze test,then sacrificed and the mRNA level of Sirt1 and MAO-A in the hippocampus were determined by real-time PCR.Results:(1) The rat in PTSD groupshowed significant less time in the central field(44.94± 13.66,16.86± 7.12,P＜0.05)and decreased time in open arm (55.90± 26.39,23.36± 12.23,P＜0.01),simultaneously mRNA level of Sirt1 and MAO-A in hippocampus were increased(1.00± 0.07,1.90± 0.05;1.01± 0.10,1.54± 0.15,P＜0.01);(2)Electroacupuncture preconditioning reversed anxiety behaviors in rats exposed to SPS&S paradigm (16.86± 7.12,36.24± 13.28,P＜0.05;23.36± 12.23,50.14± 20.77,P ＜ 0.05);(3) Electroacupuncture precondition restored the mRNA level of Sirt1 and MAO-A in the hippocampus of PTSD rat(1.90± 0.05,1.30± 0.08,P＜0.05;1.54± 0.15,1.23± 0.15,P＜0.05).Conclusion:Electroacupuncture preconditioning reversed anxiety behaviors in rats exposed to SPS&S paradigm and restored the mRNA level of Sirt1 and MAO-A in the hippocampus.
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