藏红花酸抗小鼠肝纤维化的实验研究

摘要

目的:通过观察藏红花酸对肝纤维化小鼠肝组织病理学改变及p38MAPK蛋白表达的影响,探讨其抗肝纤维化作用及可能的机制.方法:36只6周龄雄性昆明小鼠随机分为3组:藏红花酸组、模型组、正常组.除正常对照组外,其余两组小鼠给予30%四氯化碳橄榄油溶液腹腔注射,首次注射5 mL/Kg,以后每次3mL/Kg,每3日1次,连续12周.同时藏红花酸组给予藏红花酸5mg/Kg灌胃,每日1次,连续12周.实验过程中,观察各组小鼠的一般状态.12周末处死小鼠,肝组织切片行HE、Masson染色,显微镜下观察病理改变,免疫组化检测小鼠肝组织p38MAPK蛋白的表达.结果:实验过程中,模型组小鼠一般状态较差,体重增长缓慢;藏红花酸组小鼠一般状态尚可,体重较模型组增长明显(P<0.05).肝组织HE、Masson染色结果显示:模型组小鼠肝纤维化程度较重(造模成功),与其相比,藏红花酸组小鼠肝纤维化程度有所改善,差异有统计学意义.与模型组相比,藏红花酸组肝组织p38MAPK表达显著下降(P<0.05).结论:藏红花酸能有效减轻小鼠肝损伤及纤肝维化程度,其抗肝纤维化的机制可能与下调p38MAPK蛋白的表达有关.%Objective:To explore the effect and mechanism of crocetin on hepatic fibrosis by detecting the expression of p38MAPK protetin and liver tissue pathological changes in mice with liver fibrosis.Methods:36 six-week old male kunming mice were randomly divided into 3 groups:the crocetin group,model group and normal group.Except the mice of normal group,the others were given 30 % carbon tetrachloride olive oil solution by intraperitoneal injection to induce the mice models of hepatic fibrosis once every three days.And crocetin group mice were treated with crocetin by gavage everyday.During the experiment,the general condition of mice was recorded,such as the weight.After 12 weeks,the mice were sacrificed.Then theit liver tissue were collected for study.The liver pathological changes of mice were observed by HE and Masson staining.The expression of p38MAPK protein of mice liver tissue were detected by Immunohistochemistry (IHC).Results:During the experiment the general state of model group was gradually getting worse.Mice in crocetin group got better general state.Compared with the model group,the weight of crocetin group mice was increased (P< 0.05).The difference was statistically significant (P < 0.05).Liver tissue pathology results showed the degree of liver inflammation and fibrosis in model group was the heaviest and treatment with crocetin have a obvious improvement in the pathological changes.Compared with the model group,the expression of p38MAPK protein of mice liver tissue in Crocetin group was reduced significantly (P < 0.05).Conclusions:Crocetin could reduce the degree of liver injury and liver fibrosis of mice significantly,which might be related to the reduce the p38MAPK protein expression.

著录项

  • 来源
    《现代生物医学进展》|2017年第028期|5432-5435,5577|共5页
  • 作者单位

    哈尔滨医科大学附属第四医院感染科 黑龙江哈尔滨150001;

    哈尔滨医科大学附属第四医院感染科 黑龙江哈尔滨150001;

    哈尔滨医科大学附属第四医院感染科 黑龙江哈尔滨150001;

    哈尔滨医科大学附属第四医院感染科 黑龙江哈尔滨150001;

    哈尔滨医科大学附属第四医院感染科 黑龙江哈尔滨150001;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 R285.5;Q95-3;R575.2;
  • 关键词

    藏红花酸; 肝纤维化; p38MAPK;

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