3种不同的包埋材料固定化YS-9412-130菌体细胞，进行半连续发酵。结果表明，用2.5％卡拉胶固定化细胞，产酶效率最高；添加明胶对产酶不利而添加豆饼粉与玉米粉后，酶活力有显著提高；卡拉胶固定化细胞发酵液中游离菌体浓度随发酵时间变化略有上升，总体水平较低。发酵液酶活随菌体浓度的增大而呈上升趋势；固定化细胞半连续发酵效率远高于游离细胞分批发酵的效率。%The cells of F. Yellowsea YS-9412-130 immobilized with three different imbedding materials are fermented semi-continuously. The result shows that the enzyme-producing rate reaches the highest value when cells are immobilized with 2.5% carrageenan, adding soy bean cake meal and cornsteep meal can improve the enzyme activity greatly, while adding gelalin is bad to enzyme activity; the concentration of free cell in the fermented broth of carrageenan-immobilized cells arises slightly with fermentation time going, however the level in general is low; the enzyme activity of fermented broth shows a rising trend with the increase of the cells concentration; the efficiency of semi-continuous fermentation of immobilized cells is much higher than that of batch-fermentation with free cells.
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