超声波酶解法提取鼠尾藻多糖

摘要

The extraction of polysaccharides from Sargassum thunbergii by cellulase hy-drolysis and ultrasound was studied. Effects of four factors including ultrasonic power, cellula-seconcentration, extraction time and extraction temperature were discussed. On the basis of single factor analysis, the optimal operation variables were described through orthogonal experiment. Results indicated that influence of factors on the extraction were in the following order: extraction time, extraction temperature, ultrasonic power, and cellulase concentration. The optimal condition for extraction were: 300 W ultrasonic power, 3% cellulase, 50 °C and 15 min. Moreover, the cellulase addition increased the extraction rate by 37. 03% compared with the control group.%采用超声波及纤维素酶解法提取鼠尾藻多糖,探讨了功率、酶量、温度、时间对鼠尾藻多糖制备的影响,运用单因素设计正交试验,得出4种因素对鼠尾藻多糖提取的影响程度由大到小分别是时间、温度、超声波功率、酶量,优化得到最佳提取条件为超声波功率300 W、酶量3％、温度50℃、时间15 min；并通过对比分析,得出添加纤维素酶比对照组提取率提高37.03％.