高植物蛋白饲料中以低分子水解蛋白替代鱼粉对牙鲆生长性能及非特异性免疫的影响

摘要

以初始平均体重为38.80±0.11g的牙鲆Paralichth ys olivaceus为实验对象,研究在高植物蛋白饲料中用低分子水解蛋白替代鱼粉对牙鲆生长、饲料利用及非特异性免疫指标的影响.分别以不同水解鱼蛋白替代总蛋白的0(FM1为负对照,含豆粕55％、鱼粉19％)、0(FM2为正对照,含豆粕45％、鱼粉25％)、11％(FPH11)、16％(FPH16)、21％ (FPH21)、26％ (FPH26)制得7组等氮等能饲料.实验结果表明,用低分子水解鱼蛋白替代总蛋白11％组的特定生长率显著高于负对照组(P＜0.05),且与正对照组无显著性差异(P＞0.05)；FPH11、FPH16、FPH21和FPH26组的摄食率显著高于负对照组(P＜0.05)；替代总蛋白11％组的蛋白沉积率显著高于负对照组(P＜0.05),与正对照组相等(P＞0.05)；替代水解蛋白各组的蛋白质消化率均显著性高于负对照组(P＜0.05),与正对照组无显著性差异(P＞0.05)；替代水解蛋白各组的SOD活力都显著高于负对照组(P＜0.05),且替代总蛋白11％组显著性高于正对照组；FPH6、FPH11、FPH16组的总抗氧化能力显著高于负对照组但显著低于正对照组(P＜0.05).%A 63-day feeding experiment was conducted to evaluate the effects of fish protein hydrolysate as feed ingredient in high plant-protein diets on growth performance and nonspecific immunity for Japanese flounder,Paralichthys olivaceus.Fish protein hydrolysates respectively replaced 0 (FM],negative control,containing 55% soybean meal and 19% fish meal),0 (FM2,positive control,containing 45% soybean meal and 25% fish meal),6% (FPH6),11% (FPH11),16% (FPH16).21% (FPH21),and 26% (FPH26)of the total protein.The results showed that specific growth rate and protein retention of fish fed with FPH11 was significantly higher than FM,(P<0.05),but was not significantly different from FM2 (P> 0.05).For protein digestibility,all the hydrolyzed protein diets were significantly higher than FM,(P<0.05),but was not significantly different from FM2(P>0.05).Feeding rates of FPH11,FPH16,FPH21and FPH26 were significantly higher than FM2(P<0.05).The relative activities of acid phosphatase (ACP) in fish fed with FM1 was significantly lower than FPH26(P <0.05).SOD relative activities in all the hydrolyzed protein diet were higher than FM1 (P< 0.05),and SOD relative activities in FPH11was significantly higher than FM2(P<0.05).Total antioxidative capacity of FPH6,FPHnand FPH16 were significantly higher than FM1 and lower than FM2(P<0.05).