乳制品-酵母益生元对星斑川鲽幼鱼生长性能和抗氧化能力的影响

摘要

An 8-week feeding trial was conducted to study the effect of dairy-yeast prebiotic (Grobiotic-A) on the survival rate,growth performance and antioxidant capacity of juvenile starry flounder Platichtahys stellatus.A total of 540 fish at body weight of 15.05±0.04 g were randomly allocated into six groups with three replicates and each replicate contained 30 fish.The fish were fed diets supplemented with 0,0.4％,0.8％,1.2％,1.6％ and 2.0％ dairyyeast prebiotic,respectively.The results are shown as follows:1) weight gain rate (WGR),specific growth rate (SGR),feed intake (FI),protein efficiency rate (PER) and condition factor (CF) increased whereas feed conversion ratio (FCR) decreased significantly (P＜0.05) with the supplementation of dairy-yeast prebiotic in diets.However,there were no statistical differences in viscerosomatic index (VSI),hepatosomatic index (HSI) (P＞0.05) ; 2) compared with the control,the activity of catalase (CAT) and total anti-oxidation capability (T-AOC) in serum and liver,and superoxide dismutase (SOD) and anti-superoxide anion activities (T-AOC) in serum were significantly increased (P＜0.05),while the malondialdehyde (MDA) content in serum and liver of fish fed diets supplemented with dairy-yeast prebiotic was significantly decreased (P＜0.05).These results indicated that dairy-yeast prebiotic positively influenced the growth performance and anti-oxidation of juvenile starry flounder in this experiment.Quadratic regression of SGR indicated that the optimal supplementation level of dairy-yeast prebiotic in juvenile starry flounder diet was 1.32 ％.%选取平均初始体重为15.05±0.04g的星斑川鲽幼鱼540尾,随机分成6组,每组3个重复,分别饲喂添加0、0.4％、0.8％、1.2％、1.6％和2.0％乳制品-酵母益生元的试验饲料,实验期56d.结果表明:1)饲料中添加乳制品-酵母益生元可显著提高星斑川鲽幼鱼的增重率(WGR)、特定生长率(SGR)、摄食率(DFI)、蛋白质效率(PER)及肥满度(CF)(P＜0.05),显著降低饲料系数(FCR)(P＜0.05),但对星斑川鲽幼鱼的脏体比(VSI)、肝体比(HSI)无显著影响(P＞0.05).2)饲料中添加乳制品-酵母益生元显著提高了星斑川鲽幼鱼血清过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)、抗超氧阴离子自由基(O2-·)的活性和总抗氧化能力(T-AOC) (P＜0.05),显著提高星斑川鲽幼鱼肝脏中总超氧化物歧化酶(T-SOD)活性和总抗氧化能力(T-AOC) (P＜0.05),同时显著降低了血清和肝脏中丙二醛(MDA)的含量(P＜0.05).本研究条件下,饲料中添加乳制品-酵母益生元可提高星斑川鲽幼鱼的生长性能及抗氧化能力,以特定生长率为评价指标,二次回归分析得出星斑川鲽幼鱼饲料中乳制品-酵母益生元的适宜添加量为1.32％.