To test the high-temperature tolerance of turbot, young fish of different turbotrnF2 families was studied through heat shock and being cultured in high temperature seawater. Experiment A:50-day post hatching(dph) young fish from F2 families (year 2010) was heat-shocked acutely in 30℃ seawater for 2 hours, then transfered into normal saewater and the survival rate was recorded for the next 48h. Experiment B: lOOdph youngfish from F2 families (year 2009 and 2010) was reared in high temperature seawater with the water temperature being raised continuously at 2℃/day. The survival rates of young fish in each family were recorded every 12h. Twenty F2 families and 11 broodstocks were selected by the survival rate phenotype post heat shock and high temperature culture. The 20 F2 families and 11 broodstocks of turbot with high-temperature tolerance are expected to become part of the core population for breeding.%通过高温刺激和高温水养殖幼鱼的方法研究了大菱鲆不同子二代(F2)家系耐高温能力.对2010年建立的36个子二代(F2)家系50日龄幼鱼,记录使用30℃高温海水进行突变热刺激2h后48h的死亡率；对2009年和2010年所建立的22个和36个大菱鲆F2家系100日龄幼鱼,按照2℃/d速率将养殖温度分别由21.0℃和14.8℃逐步升温至27.0℃和25.0℃,记录不同家系在不同时间的死亡情况.根据热激后存活率表型筛选出高温耐受力强的20个F2家系和11尾F1亲鱼,所筛选出的亲鱼和子代家系有望成为核心育种群体构建的组成部分.
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