Objective:To capture the fingerprint marker of advanced malignant neoplasm when pain occurs. Methods:Chose 24 patients with advanced malignant neoplasm confirmed by pathology or cytology,collected peripheral venous blood on an empty stomach,followed up till three months later,they were divided into 2 groups according to the pain occurrence,pain group(9 cases)and painless group(15 cases),by SELDI - TOF - MS(surface - enhanced laser desorption/ ionization time -of - flight mass spectrometry)technique and CM10 proteinchip,these serum proteomic fingerprints were memorized. Results:There were 6 significant different protein fingerprints between painless and pain group,M/ Z values were 2 755,8 678,8 813, 11 361,11 447,11 605. Compared with painless group,M/ Z2755,11 361,11 447,11 605 were up - regulated expressed,and 8 678,8 813 down - regulated expressed. We draw the following results from further analysis:M/ Z 2 755,8 678,8 813 were pres-ent between the two groups,the median value was 6,2,3 in the pain group,and 2,6,10 in the painless group. In addtion,M/ Z 11 361,11 447,11 605 in positive expression of pain,painless group were negative. Conclusion:The protein fingerprints of M/Z2755 high protein expression,M/ Z8 678,8 813 lower expression,M/ Z11 361,11 447,11 605 positive expression can exist as the special fingerprint marker of cancer pain.%目的：借助表面增强激光解吸电离飞行时间质谱（SELDI - TOF - MS）技术捕获晚期恶性肿瘤癌痛发生时的指纹标记。方法：选择24例经病理学或细胞学确诊的晚期恶性肿瘤患者，清晨采集空腹末梢静脉血，采血后观察3个月。将3个月内发生癌痛的患者的血清样本归为癌痛组（9例），3个月内未发生癌痛的血清样本归为无痛组（15例），应用 CM10弱阳离子芯片结合 SELDI - TOF - MS 技术检测两组患者血清样本的蛋白质谱。结果：无痛组与癌痛组相比有6个蛋白质峰的差异有统计学意义（ P ﹤0.05），蛋白质质荷比（ M / Z）分别为2755，8678，8813，11361，11447，11605。其中与无痛组相比，癌痛组上调的峰 M/ Z 为2755，11361，11447，11605，下调的峰 M/ Z 为8678和8813。进一步对所有患者的蛋白质指纹图谱进行分析，得出：M/ Z 为2755，8678，8813的蛋白质组在两组之间都存在，癌痛组其丰度中位值分别为6，2，3，无痛组中其丰度中位值分别为2，6，10，而 M/ Z 为11361，11447，11605在癌痛组中呈阳性表达，无痛组则呈阴性表达。结论：蛋白质 M/ Z 2755高表达，M/ Z 8678，8813低表达，M/ Z 11361，11447，11605呈阳性表达，可以作为癌痛发生的特异指纹标记。
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