苦芪滴丸中氧化槐果碱在大鼠血浆中的药代动力学研究

摘要

Objective To establish an HPLC method for the determination of oxysophocarpin in rat plasma, and study the pharmacokinetics of oxysophocarpin in Kuqi drop pills. Methods The analytical column was Inertsil-NH2 (150 mm × 4. 6 mm,5 μm); mobile phase:acetonitrile-ethanol-3% phosphoric acid water (86∶8∶6);flow rate:1. 0 mL/min; detection wavelength: 220 nm; injection volume: 20 μL. Results The linear concentration range of plasma oxysophocarpin was 0. 20~20 μg/mL,r=0. 993,the lower limits of quantificati were 0. 20 μg/mL. The intra-day and inter-day precision,accuracy,extraction recoveries met the requirement. Six rats were fed by gavage administra-tion of 8. 5 mg/kg Kuqi drop pills,the plasma oxysophocarpin Tmax was (4. 03 ± 0. 72)h,Cmax was (4. 00 ± 0. 54)ng/mL,t1/2 was ( 0. 053 ± 0. 66 ) h, AUC0-t was ( 30. 70 ± 0. 54 ) μg·h/mL, AUC0-∞ was ( 30. 8 ± 0. 32 ) ng·h/mL. Conclusion The method is simple,rapid,accurate,specific,it can be used for the pharmacokinetic study of oxyso-phocarpin in Kuqi drop pills.%目的：建立测定血浆中氧化槐果碱浓度的高效液相色谱法,并用此方法进行苦芪滴丸中氧化槐果碱的药代动力学研究。方法色谱柱为Inertsil-NH2(150 mm ×4．6 mm,5μm)；流动相：乙腈-无水乙醇-3%磷酸水(86∶8∶6)；流速：1．0 mL/min；检测波长：220 nm；进样量：20μL。结果血浆中氧化槐果碱的线性范围：0．20~20μg/mL,r=0．993,最低检测限为0．20μg/mL。日内、日间精密度及准确度、提取回收率符合要求。6只大鼠灌胃给药8．5 mg/kg苦芪滴丸后,血浆中氧化槐果碱Tmax为(4．03±0．72)h,Cmax为(4．00±0．54)ng/mL, t1/2为(0．05±0．66)h,采用梯形法计算,AUC0-t为(30．70±0．54)μg·h/mL,AUC0-∞为(30．8±0．32)ng·h/mL。结论本方法简便、快速、准确、专属性强,可用于苦芪滴丸中氧化槐果碱的药代动力学研究。