Objective To investigate the effect of isoliquiritigenin (ISO) on the expression of endothelial protein C receptor (EPCR) in renal tubular epithelial cells of rats with hemorrhagic shock and its possible mechanism. Methods Seventy-five adult healthy SD rats were randomly divided into 5 groups: blank control group (group C), sham operation group (group S), hemorrhagic shock group (group HS), Ringer''s fluid resuscitation group (group R) and ISO group (group I). Group R was treated with Ringer''s solution after the animal model of hemorrhagic shock was established successfully. Group I was treated with ISO (20 μg/mL) and Ringer''s solution. The rats were sacrificed at 6 h after the shock and the serum and renal tissue were collected. The levels of serum urea nitrogen and creatinine were measured by automatic biochemical analyzer. The degree of renal injury was detected by immunohistochemistry. RTPCR and Western blot were used to detect the expression of EPCR in renal tissue. Results Compared with group C and group S, the levels of serum urea nitrogen and creatinine, the expression of EPCR in renal tissue and the damage degree of renal tissue in group HS were significantly increased (P＜0. 05). The levels of serum urea nitrogen and creatinine, the expression of EPCR in renal tissue and the damage degree of renal tissue in group I and group R decreased significantly, the difference being statistically significant (P＜0. 05); the decrease degree of group I was more obvious (P＜0. 05), and compared with group R, the difference being statistically significant (P＜0. 05). Conclusion The levels of serum urea nitrogen and creatinine and the expression of EPCR in renal damage of hemorrhagic shock rats are related to renal damage in hemorrhagic shock. ISO can inhibit the expression of EPCR in renal tissue, thus playing a role in the protection of renal function.%目的 探讨异甘草素对失血性休克大鼠动物模型肾脏内皮细胞蛋白C 受体 (EPCR) 表达的影响及其可能的机制.方法成年健康SD 大鼠75 只, 随机分为5 组:空白对照组 (C 组) 、假手术组 (S 组) 、失血性休克组 (HS 组) 、林格液复苏组 (R 组) 和异甘草素复苏组 (I 组), 每组15 只.建立失血性休克大鼠动物模型成功后, R 组采用林格液复苏; I 组采用异甘草素 (20 μg/mL) 和林格液组成的液体复苏.休克后6 h 分别处死大鼠, 留取外周血清和肾组织, 采用全自动生化分析仪检测血清尿素氮和肌酐水平, 采用免疫组化检测肾脏的损伤程度, 采用RT-PCR 法和Western blot 法检测肾脏组织EPCR 的表达.结果 与C 组和S 组比较, HS 组大鼠血清尿素氮和肌酐水平、肾组织EPCR 的表达以及肾脏组织的损伤程度明显升高 (P＜0. 05); R 组和I 组复苏后血清尿素氮和肌酐水平、肾组织EPCR 的表达以及肾脏组织的损伤程度均明显下降, 差异有统计学意义 (P＜0. 05); 其中, I组的下降程度更加明显 (P＜0. 05), 与R 组比较差异有统计学意义 (P＜0. 05).结论 失血性休克大鼠的血清尿素氮和肌酐水平, 以及肾脏组织EPCR 表达增加, 这与失血性休克时肾脏损伤有关.异甘草素可以抑制肾组织 EPCR 的表达, 从而起到保护肾脏功能的作用.
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