B/T淋巴细胞弱化因子在急性哮喘小鼠模型CD4+T淋巴细胞表达的研究

摘要

目的 研究负向共刺激受体B/T淋巴细胞弱化因子(Band T lymphocyte attenuator,BTLA)在急性哮喘小鼠模型脾脏CD4+T淋巴细胞中的表达特点及地塞米松对BTLA表达的影响.方法 18只BALB/C6小鼠按随机数字表法分为空白对照组、哮喘模型组、地塞米松治疗组.OVA联合AL(OH)3第1和第8天分别致敏和第15～ 17天激发,建立急性哮喘模型.HE染色观察3组气道炎症浸润情况；Elisa检测血清IL-4和IFN-γ；流式细胞术检测小鼠脾脏CD4+T淋巴细胞BTLA表达变化.结果 HE染色哮喘组肺部小气道和血管周围炎症细胞浸润(EOS)明显,上皮细胞增生；流式细胞仪检测哮喘组脾脏CD4+T淋巴细胞表面BTLA表达较对照组明显增高(P＜0.01),BTLA在地塞米松治疗组比哮喘模型组和空白对照组均增高,差异有统计学意义(P＜0.05).结论 BTLA在急性哮喘模型表达上调参与炎症调控；地塞米松能进一步上调BTLA表达,抑制气道炎症.%Objective To investigate the expression of B and T lymphocyte attenuator (BTLA) in CD4+ T lymphocytes of a mouse acute asthma model and the influence of DXM on the expression.Methods Mice were sensitized by intraperitoneal injection of OVA and aluminium in saline and challenged by nebulization of 2.5％ OVA on days 15 ～ 17.HE stating was performed to examine airway inflammation of control,asthma and DXM groups.Serum concentrations of IL-4 and IFN-γ were assayed by ELISA kits.Expression of BTLA in the membrane of CD4+lymphocytes of mice was analyzed by flow cytometry.Results Prominent perivascular and peribronchial eosinophilic inflammatory cell infiltration,along with subepithelia goblet cell hyperplasia,was observed in the asthma mice.The highest expression of BTLA in the membrane of CD4+lymphocytes was observed by flow cytometry in the DXM group,followed by the asthma group,and the lowest in the control group (P ＜ 0.05).Conclusion Expression of BTLA is increased in acute asthma mice model,and could be further up-regulated by DXM.