系统性红斑狼疮患者外周血T淋巴细胞亚群的临床分析

摘要

目的 了解系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血T淋巴细胞的变化,探讨T淋巴细胞与SLE的疾病活动度及24小时尿蛋白的关系.方法 采用流式细胞仪对76例SLE患者(低度活动组与高度活动组)及同期36例健康女性(健康对照组)的外周血淋巴细胞亚群进行检测.结果 与健康女性比较,75.0%的SLE患者存在淋巴细胞亚群的异常,表现为CD3+CD4+细胞减低(P ＜ 0.01),CD3+CD8+细胞增高(P ＜ 0.01),CD3+CD4+/ CD3+CD8+比值减低,CD3+CD4+/ CD3+CD8+比值减低中59.6%出现倒置(P ＜ 0.01);T细胞亚群的异常与狼疮活动度、24小时尿蛋白、血沉、补体C3、C4有明显相关性(P ＜ 0.05).结论 T细胞亚群检测不但能提示SLE患者细胞免疫功能受损,还是对SLE疾病活动及严重程度进行判断的参考指标.%Objective To examine the changes of peripheral blood T lymphocyte subsets in the patients with systemic lupus erythematosus (SLE) ,and to investigate the relationships between T lymphocyte,disease activity and 24-hour urine protein excretion. Methods Peripheral blood T lymphocyte subsets of 76 patients with systemic lupus erythematosus (SLE) and 36 healthy women were determined by flow cytometry. Results Compared with healthy women, there were abnormalities of peripheral blood T lymphocyte subsets in 75.0% of SLE patients. The CD3 + CD4+ cells were lower(P < 0.01) ,and the CD3+CD8+ cells were higher(P < 0.01). The ratios of CD3 + CD4+ cells/CD3 + CD8+ cells were lower,59. 6% of which were inverted (P < 0. 01). The abnormal T cell subsets significantly correlated to the disease activity,24-hour urine protein excretion,erythrocyte sedimentation rate and complements C3,C4 in SLE patients. Conclusions Abnormal T cell subsets may suggest that cellular immune functions are impaired in SLE patients, and the abnormality of T cell subsets is a helpful parameter for lupus activity and severity.