淫羊藿苷对人外周血内皮祖细胞数量及功能的影响

摘要

Objective To explore the effects of icariin on the numbers and the function of endothelial progenitor cells ( EPCs) isolated from peripheral blood in human. Methods EPCs were isolated from peripheral blood of human by the method of density gradient centrifugation. According to the icariin concentration ( 0, 10, 20, 50 μmol/L ) added to culture medium, they were divided into control group, low dose group, middle dose group and high dose group respectively. Those groups were incubated for 48 hours, then the number of EPCs and their impact on cell function were observed. Re⁃sults Compared with control group, it was observed in icariin treatment group that the number of EPCs in peripheral blood and the ability of adhesion, migration, proliferation and angiogenesis in vitro increased obviously ( P<0�05) ,and high dose group(50 μmol/L) was the most significant. Conclusions Icariin can significantly increase the number of EPCs in hu⁃man peripheral blood, and improve its capabilities of proliferation, adhesion, migration and angiogenesis.%目的探讨淫羊藿苷对体外培养的人外周血内皮祖细胞（ EPCs）的数量及相关功能的影响。方法采用密度梯度离心法分离出人外周血的EPCs，将淫羊藿苷加入相应培养基，并根据加入培养基中淫羊藿苷的浓度0、10、20、50μmol／L分为对照组、低剂量组、中剂量组和高剂量组，继续培养细胞48 h。然后检测各组EPCs数量及功能的变化并进行比较。结果淫羊藿苷干预组的外周血EPCs的数量以及增殖、迁移、黏附能力和血管生成能力与对照组相比均有明显提高，差异有统计学意义（ P＜0�05），并且其中高剂量组（50μmol／L）效果最为显著（ P＜0�05）。结论淫羊藿苷能明显增加人外周血EPCs的数量并改善EPCs的增殖、黏附、迁移以及血管生成能力等相关功能。