Fusarium boothii 特异性 PCR 检测方法的建立与应用

摘要

A pair of species-specific primers F-Fg/R-Fg was designed for detection of Fusarium boothii based on reductase-like gene sequences of Fusarium spp.A total of 35 strains of Fusarium were tested by using the primers, the results showed that the F-Fg/R-Fg were of high specificity,since only a single product of about 300 bp was amplified from Fusarium boothii genomic DNA.The sensitivity of the detection was 50 pg genomic DNA per 20μL PCR reaction volume.%根据报道的 Fusarium spp．的 reductase-like 基因序列，设计合成了1对用于 Fusarium boothii 的特异性检测引物F-Fg／R-Fg。利用该对引物对包括 F．boothii 在内的35株镰刀菌的基因组 DNA 进行了扩增。结果表明：该引物特异性强，仅从 F．boothii 基因组 DNA 中扩增出300 bp 左右的特异性条带，其他参照菌株及阴性对照均无任何条带；灵敏度验证结果表明，该检测法可以检测出50 pg F．boothii 基因组 DNA。