Development of RAPD-based SCAR Marker for Rapid Identification of Trichoderrna harzianurn

摘要

Based on previous morphological classification and RAPD analysis,we recovered and sequenced genomic DNA of a specific fragment obtained from No.45 Trichoderma harzianumstrain in RAPD amplification.A pair of specific primers was synthesized and the RAPD markers were successfully converted into SCAR markers.The SCAR molecular marker exhibited the specificity that could differ T.harzianumfrom other Trichoderma strains.This pair of molecular primers was used for specific PCR amplification of 45 Trichoderma strains,and T.harzianumstrains could amplify a 779 bp DNA band while other strains had no amplification products.