[Objective] To develop biological control resources of endophytic bacteria in wheat,the bacteria with antagonistic effect against Fusarium graminearum were screened and their antagonistic activity was studied. [Method] The endophytic bacteria of wheat with antagonistic effect were screened using plate confrontation method; the antagonistic bacteria were identified through physiological and biochemical characteristics and 16 S r DNA sequence analysis; the antagonistic spectrum of antagonistic bacteria was studied using plate confrontation method. [Result]The antagonistic bacterium XY-3 was isolated from wheat leaves,and was identified as Bacillus amyloliquefaciens according to the physiological and biochemical characteristics and 16 S r DNA sequence analysis. [Conclusion] XY-3 had antagonistic effect against four kinds of common pathogenic fungi,presenting broad spectrum antagonistic activity against plant pathogenic fungi.
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