3D printing-based drug-loaded implanted prosthesis to prevent breast cancer recurrence post-conserving surgery

摘要

Systemic chemotherapy of breast cancer is commonly delivered as a large dose and has toxic side effects.Local chemotherapy would overcome the shortcomings of systemic reconstruction and could play an important role in breast cancer surgery according to personalized demand.The application of three-dimensional(3D)printing technology makes personalized customization possible.We designed and prepared a prosthesis containing paclitaxel(PTX)and doxorubicin(DOX)microspheres(PPDM)based on 3D printing to prevent tumor recurrence and metastasis after breast conserving surgery.Polydimethysiloxane has good biocompatibility and was used as a drug carrier in this study.The average particle size of the PTX and DOX microspheres were approximately 3.1μm and 2.2μm,respectively.The drug loading of PTX and DOX microspheres was 4.2% and 2.1%,respectively.In vitro drug release studies demonstrated that the 3D-printed prosthesis loaded with PTX and DOXmicrospheres could release the drugs continuously for more than 3 weeks and thereby suppress cancer recurrence with reduced side effects.The PTX and DOX microspheres not only exerted a synergistic effect,but also achieved a good sustained release effect.In vivo evaluation showed that the PPDM could effectively inhibit breast cancer recurrence andmetastasis inmice with breast cancer.PPDM are expected to achieve postoperative chemotherapy for breast cancer and be highly efficient to prevent local breast cancer recurrence and metastasis.