HPLC法同时测定消氮颗粒中芦荟大黄素大黄酸大黄素大黄酚和大黄素甲醚五种成分的含量

摘要

目的：用HPLC法同时测定消氮颗粒中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5种成分的含量。方法选用VP-ODS C18色谱柱（250 mm ×4.6 mm，5μm），流动相：甲醇-0.1%磷酸（85：15），检测波长：254 nm，流速：1.0 ml· min-1，柱温：30℃。结果芦荟大黄素、大黄素、大黄酸、大黄酚均在1.60~8.00μg·ml-1范围内浓度与峰面积线性关系良好，大黄素甲醚在0.86~4.8μg·ml-1范围内线性关系良好，样品中各成分的平均回收率分别为芦荟大黄素99.07%（ RSD为1.81%），大黄酸98.96%（RSD为1.07%），大黄素98.65%（RSD为1.33%），大黄酚99.27%（RSD为1.87%）和大黄素甲醚98.33%（RSD为1.07%），n ＝6。结论本文方法快速、灵敏、准确，样品处理简便易行。%Objective To determine the content of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xiaodan granules. Methods The HPLC system consisted of VP-ODS column(250 mm × 4. 6 mm,5 μm)with methanol-0. 1% phosphoric acid(85:15)as the mobile phase. The detection wavelength was 254 nm,flow rate 1 . 0 ml·min-1 and column temperature 30 ℃. Results The calibration curve was linear over the range of 1 . 60 -8. 00 μg·ml-1( r =0. 9999)for aloe-emodin,emodin,rhein and chrysophanol,but 0. 86-4. 80 μg·ml-1 for phy-scion. The mean recovery was 99. 07%( RSD=1. 81%)for aloe-emodin,98. 96%( RSD=1. 07%)for rhein, 98. 65%( RSD =1. 33%)for emodin,99. 27%( RSD =1. 87%)for chrysophanol,and 98. 33%( RSD =1. 07%)for physcion,respectively,n =6. Conclusion This method is sensitive,quick and accurate.