便携式上转换荧光试纸条检测仪的研制

摘要

A portable up-conversion fluorescent strip detector was developed to achieve the quantitative analysis of the up-conversion fluorescent strip.The performance of the detector was verified by practical measurements.The up-conversion fluorescent technology and the principle of up-conversion fluorescent strip were introduced firstly.The up-conversion fluorescent strip detector contained two critical components that were optical scanning system and electrical system,enabling the conversion of fluorescence signals to electric signals.Then,the prototype of the detector was developed and tested experimentally,therein the sampled signals detected by the strip were optimized.This apparatus proves great stability and repeatability,and the standard deviation of the detection is less than 0.004.The linear response characteristic is also excellent as the coefficient of determination of concentration standard curve reaches 0.996 3.This detector has a low power consumption and high performance with an exquisite structure,thus satisfying the requirements of quantitative analysis through upconversion fluorescent strip.%为了实现对上转换荧光试纸条的定量检测,研制了便携式上转换荧光试纸条检测仪,并通过实际测试验证了仪器的主要性能.首先,介绍了上转换荧光技术以及上转换荧光试纸条的测试原理,描述了以光学扫描检测系统和电路系统为核心的便携式荧光试纸条检测仪的设计方案,能够将试纸条的荧光信号转变为电信号并进行处理;然后,基于设计方案研制了检测仪样机,并对实际检测试纸条采集到的信号进行优化;最后,对检测仪的性能进行了测试.测试结果表明:仪器稳定、重复性好,样本检测标准差小于0.004;线性响应特性良好,拟合出的浓度标准曲线决定系数为0.996 3.该检测仪结构精巧、功耗低、性能良好,很好地满足了上转换荧光试纸条定量检测的需求.