便携式MicroRNA快速检测系统

摘要

MicroRNA(miRNA)与疾病的发生和发展密切相关,可作为疾病诊断标志物.常规检测miRNA的方法耗时较长且操作复杂,本文设计了一种便携式miRNA快速检测系统.该系统采用光电检测技术检测滚环扩增后的标志物miRNA受激发出的荧光强度,对采集到的荧光数据进行分析,得出miRNA的变化情况.实验得出便携式miRNA快速检测系统在试剂浓度为0.1～1μmol时,荧光强度线性偏倚不超过±5％,系统重复性大于95％.通过对冠心病检测标志物miR-499进行滚环扩增实验,结果表明设计的便携式miRNA快速检测系统能够快速有效地检测miRNA.%MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker.Conventional methods for the detection of miRNA are time-consuming and complex.Herein,a portable rapid miRNA detection system was designed.This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique,and analyzes the fluorescence data to obtain the changes in miRNA.Through our experiments,we observed that for reagent concentrations in the range 0.1-1 μmol,the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than ±5％,and the repeatability was ≥95％.Our roll amplification experiment involving the coronary heart disease diagnostic marker,miR-499,confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively.