紫贻贝（Mytilus edulis）养殖群体的遗传多样性及遗传结构分析

摘要

The genetic diversity and genetic structure of three cultured populations （Yantai of Shandong province, Rushan of Shandong province and Dongji of Zhejiang province） of Mytilus edulis were determined using the mitochondrial gene sequencing technology （COⅢ）. The result revealed 18 haplotypes for total 32 individuals obtained using PCR method. The haplotype diversities （Hd）, nucleotide diversities （Pi） and the average nucleotide differences （K） were 0.946, 0.0207 and 15.316, respectively, which showed high genetic diversity among populations. The fixation indices （Fst） of three populations showed that there was no genetic differentiation between Yantai and Rushan populations and significant genetic differentiation between Dongji and the other two populations.%基于线粒体DNA的COⅢ基因序列对我国沿海重要经济贝类紫贻贝3个养殖群体（烟台、乳山、东极）的遗传多样性和遗传结构进行了研究。由PCR扩增获得32个体的COⅢ基因750bp的部分序列,其多态性遗传参数统计显示,32个个体共检出18个单倍型,总群体单倍型多样性指数（Hd）为0.946,核苷酸多样性指数（Pi）为0.0207,平均核苷酸差异数（K）为15.316,三个群体均显示出较丰富的遗传多样性。遗传分化系数（Fst）表明,东极群体与烟台群体及乳山群体已明显分化,而烟台群体和乳山群体间无遗传分化,并且存在着较大的基因流。