Effects of MCI-186 on intracellular calcium ion concentration and membrane fluidity of hippocampal neurons in a rat model of Alzheimer's disease

摘要

The present study observed the effects of MCI-186 on the intracellular calcium ion concentra-tion ([Ca2+]i) and membrane fluidity of hippocampal neurons in Alzheimer's disease (AD) model rats to validate the neuroprotective effects of MCI-186.Compared with normal and sham-operated rats,the escape latency was obviously prolonged,spanning platform times were obviously reduced,[Ca2+]i was increased,and microviscosity η value was increased in AD rats.MCI-186 at 0.5 mg/mL and 0.2 mg/mL obviously shortened escape latency,increased spanning platform times and decreased [Ca2+]i and microviscosity η values in AD rats at 7 and 17 days after induction of the model.The differences in the above-mentioned indices between normal and MCI-186-treated rats were statistically significant.MCI-186 at 0.2 mg/mL yielded better curative effects than 0.5 mg/mL MCI-186.These findings suggest that MCI-186 improves learning and memory abilities in AD rats by decreasing [Ca2+]i and increasing membrane fluidity of hippocampal neurons.