美洲大蠊抗氧化活性成分研究

摘要

To study the chemical constituents from 95％ ethanol extract of Periplaneta americana.The chemical constituents were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography,0DS column chromatography and semi-preparative HPLC.Fourteen compounds were obtained from the ethyl acetate fraction of P.americana based on NMR and MS spectroscopic methods.Their structures were identified as cyclo (L-Val-L-Pro) (1),3,4-dihydroxyphenylethanol (2),protocatechuic acid (3),cyclo (L-Ile-L-Pro) (4),cyclo (L-Phe-L-Val) (5),nicotinic acid (6),hippuric acid (7),aspongpyrazine A (8),aspongopusin (9),(2R,3S)-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-hydroxyethyl-1,4-benzodioxane (10),(2R,3S)-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-(N-acetyl-2"-aminoethylene)-1,4-benzodioxane (11),cyclo (Leu-Pro) (12),1,2-dehydro-N-acetyldopamine (13) and 3,4-dihydro-8-hydroxy-2(1 H)-quinolinone(14).Among them,compounds 1,4-9 and 10-13 were isolated from the genus for the first time.The spectral data of compound 10 were reported for the first time.All compounds were evaluated for antioxidant activity.Compounds 2,3 and 13 showed potential antioxidant activity by DPPH and ABTS+ radical scavenging methods.%对美洲大蠊(Periplaneta americana)虫体95％乙醇提取物的化学成分进行研究.采用硅胶柱色谱,凝胶柱色谱,开放ODS柱色谱和半制备高效液相色谱等技术进行分离纯化,通过核磁共振、质谱等方法鉴定化合物的结构.结果从乙酸乙酯部位分离并鉴定14个化合物,分别为:环(L-缬氨酸-L-脯氨酸)二肽(1)、3,4-二羟基苯乙醇(2)、原儿茶酸(3)、环(L-异亮氨酸-L-脯氨酸)二肽(4)、环(L-苯丙氨酸-L-缬氨酸)二肽(5)、烟酸(6)、马尿酸(7)、aspongpyrazine A(8)、aspongopusin(9)、(2R,3S)-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-hydroxyethyl-1,4-benzodioxane(10)、(2R,3S)-2-(3',4'-dihydroxyphenyl)-3-acetylamino-7-(N-acetyl-2"-aminoethylene)-1,4-benzodioxane(11)、环(亮氨酸-脯氨酸)二肽(12)、1,2-二去氢-N-乙酰多巴胺(13)、3,4-二氢-8-羟基-2(1H)-喹啉酮(14).其中化合物1、4～9和10～ 13为首次从大蠊属昆虫中分离得到,化合物10的波谱数据为首次报道.对已分离的14个化合物利用DPPH和ABTS+自由基清除实验评价化合物的体外抗氧化活性,结果显示化合物2、3、13具有潜在的抗氧化活性.