阻抗谱芯片用于人眼小梁网细胞分析

摘要

The in vitro cultured human trabecular meshwork (TM) was investigated using electric impedance spectroscopy (EIS) in real time.TM is a critical determinant of intraocular pressure (IOP),the increase of which is the main cause of glaucoma.An array of interdigitated microelectrodes (IMEs) was fabricated for electric impedance sensing.An equivalent electric circuit model was proposed to study the cellular activities on the IMEs,showing that the impedance of extracellular matrix (ECM) and the cell coverage ratio have distinct signatures on the spectrum.The utility of the EIS was demonstrated by treating TM cells during their proliferation with dexamethasone (DEX).The impedance measurement at 40 kHz showed that a higher DEX concentration can induce more severe inhibition of cell proliferation.The measurement was validated by AlamarBlue assay.The measurement provided a reasonable explanation on glucocorticoid induced glaucoma,where the glucocorticoid alters the metabolic and physiological functions of TM cells by inhibiting normal cell growth and proliferation.%采用阻抗谱分析法对体外培养人眼小梁网细胞进行实时在线检测.人眼眼压过高将导致青光眼病变,而小梁网对于眼压高低至关重要.制备了叉指式阵列微电极作为阻抗传感器敏感单元.提出了分析小梁网细胞生物行为特征的等效电路模型,通过仿真分析表明该电路模型可在不同频段区分人眼小梁网细胞外基质和细胞繁殖率等阻抗参数值.采用叉指式阻抗谱芯片对经地塞米松药物处理的小梁网细胞进行了实时在线监测,结果表明40 kHz时高浓度地塞米松对小梁网细胞增殖具有较大的抑制作用,该结论与AlamarBlue试剂法得到的结论一致.阻抗测试结果为糖皮质激素诱导的青光眼提供了一种合理的解释,认为该病变与正常细胞增殖率异常有关.