海南热带雨林腐木上两个无性型子囊菌酵母新种

摘要

SWains X2WZ07-4 and G2WZ06-1 isolated from rotten wood collected flom the tropical rainforests in Hainan Island were revealed to represent two new anamorphic, ascomycetous yeast species by molecular taxonomic characterization. Sequence analysis from the 26S rRNA gene D1/D2 domain showed that strains X2WZ07-4 and G2WZ06-1 were closely related with Candida cylindracea and C llanquihuensis, respectively. The two strains differed from their closest described relatives by 2.5% to 3.9% mismatches, indicating that they represent two novel species. Since sexual states were not observed in the cultures of the two strains in various media, two anamorphic names Candida pseudocylindracea sp. nov. (type strain X2WZ07-4T = AS 2.3788T = CBS 10854T) and C wuzhishanensis sp. nov. (type strain G2WZ06-1T = AS 2.3784T = CBS 10850T) are proposed for them, respectively.%用含木糖为唯一碳源和含葡萄糖及7.6%乙醇的两种富集培养基对采自海南热带雨林腐木样品中的酵母菌进行了分离培养.对分离出的酵母菌株进行的分子分类学研究表明,其中两株酵母菌X2WZ07-4和G2WZ06-1代表两个无性型子囊菌酵母新种.大亚基(268)rRNA基因D1/D2域序列分析显示,与X2WZ07-4和G2WZ06-1亲缘关系最近的已知种分别为Candida cylindracea和C llanquihuensis.在D1/D2域,X2WZ07-4与C cylindracea模式菌株的碱基序列差异为2.5%;G2WZ06-1与C. llanquihuensis模式菌株的序列差异为3.9%,均远大于酵母菌种间在此区域的序列差异(～1%).这两个新种分别被命名为拟柱形假丝酵母Candida pseudocylindracea sp.nov.(模式菌株:X2WZ07-4T=AS 2.3788T=CBS 10854T)和五指山假丝酵母Candida wuzhishanensis sp.nov.(模式菌株:G2WZ06-1T=AS 2.3784T=CBS 10850T).