采用EF-1α序列及气生菌丝上的孢子形态特征对九州镰孢菌、拟枝孢镰孢菌和厚垣镰孢菌的鉴定

摘要

对九州镰孢菌Fusarium kyushuense、厚垣镰孢菌F.chlamydosporum和拟枝孢镰孢菌F.sporotrichioides在气生菌丝上产生的孢子进行了比较.九州镰孢菌在气生菌丝上产生多隔孢子(即中型分生孢子)；厚垣镰孢菌在气生菌丝上产生的主要是0隔针叶状分生孢子；拟枝孢镰孢菌在气生菌丝上产生两种类型的分生孢子:芜菁形、单胞分生孢子以及椭圆形、0-1隔的分生孢子.多隔的气生孢子(中型分生孢子)在厚垣镰孢菌和拟枝孢镰孢菌这两个种中偶尔可以观察到,但是不应作为对这两个种进行鉴定的主要特征.EF-1a序列的最小进化树分析表明,来自中国的九州镰孢菌具有独特的单核苷酸多态性,与来自日本的菌株间存在差异.来自中国的厚垣镰孢菌分离株在厚垣镰孢菌复合种(FCSC)中形成独特的菌系.%Conidia produced in aerial mycelium of Fusarium kyushuense,F.chlamydosporum and F.sporotrichioides were compared.F.kyushuense produced multi-septate conidia in the aerial mycelium (mesoconidia).On the contrary,conidia in the aerial mycelium of F.chlamydosporum were mainly 0-septate and spindle-shaped.F.sporotrichioides produced two types of conidia in aerial mycelium:napiform and 0-septate and elliptical and 0- 1-septate.Multi-scptate aerial conidia (mesoconidia) were occasionally observed in F.chlamydosporum and F.sporotrichioides,and they should not be treated as the key characteristics for their identification.The EF-1α sequence analysis shows that Chinese isolates of F.kyushuense possess unique single nucleotide polymorphisms,distinguishing from Japanese isolates.The isolates of F.chlamydosporum isolated from China shows a separated lineage in F.chlamydosporum species complex (FCSC).