应用两对引物,从对多菌灵具有耐药性的链格孢属3个真菌中扩增了与多菌灵耐药性相关的β-微管蛋白基因,基因长度分别为1,419bp(瓜链格孢),1,426bp(茄链格孢),1,419bp(链格孢),均包含3个内含子,编码398个氨基酸.3个链格孢属真菌与其他对多菌灵敏感的植物病原真菌β-微管蛋白氨基酸具有高度的同源性.但是,3个真菌167位氨基酸均为酪氨酸,而其他对多菌灵敏感的真菌167位均为苯丙氨酸,研究结果表明链格孢属真菌对多菌灵的耐药性可能与167位酪氨酸有关.%The β-tubulin genes from three Alternaria species which are tolerant to high concentration of carbendazim were amplified with two pairs of primers and sequenced. The lengths of three genes are 1,419bp for Alternaria cucumerina, 1,426bp for Alternaria solani, and 1,419bp for Alternaria alternata respectively. All genes have three introns and encode proteins with 398 amino acids. The protein sequences of β-tubulins of three Alternaria species are highly homologous to those of other plant pathogenic fungi that are sensitive to carbendazim. The main difference is that the 167th amino acid of P-tubulins in Ahernaria is the residue Tyr instead of Phe, which is commonly found at the same position of P-tubulins in other fungi. These results suggest that carbendazim resistance of the three Alternaria species probably attributes to Tyr167.
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