COI基因作为丛赤壳科真菌DNA条形码的测试

摘要

以丛赤壳科33种76个菌株为材料,探讨COI基因作为该科DNA条形码的可能性.结果表明,该DNA片段存在较多内含子,为了获取某些种的短片段,需设计许多引物,PCR扩增与测序成功率低,难以达到便捷、快速的物种鉴定的目的.因此,COI不宜作为丛赤壳科的DNA条形码.对已获得的少数片段进行分析表明,该基因对丛赤壳科部分种具有较强的物种鉴别力.%COI gene was tested with 76 strains of 33 species of the nectriaceous fungi to investigate its feasibility of serving as a DNA barcode. The results indicated that introns are present in some species, which made it difficult for PCR amplification and sequencing. COI gene is not a good DNA barcode for this group. Analysis of the obtained COI sequences showed that this gene may have the capacity to distinguish species of the nectriaceous fungi.