小囊网盘衣真菌新色原酮类次级代谢产物初步研究

摘要

Lichens are symbiotic ecosystem of mostly ascomycetous fungi (mycobiont) and photosynthetic partners (photobiont) which are either green algae or cyanobacteria.Using cultured separated lichenized fungus Sarcogyne asciparva as material,sarcasciols A-H (1-8),eight new isoprenylated chromone derivatives have been isolated from scale-up culture.The structures of 1-8 were elucidated by mass spectra and nuclear magnetic resonance experiments.The absolute configuration of C-6 in 1 was assigned by application of the modified Mosher's method.The antibacterial activity of compounds 1-8 was revalued by 96-well plate method.%地衣是地衣型真菌与相应藻类或蓝细菌之间紧密而稳定的独特的共生生态系统.本实验以分离自小囊网盘衣中的地衣型小囊网盘衣真菌Sarcogyne asciparva为材料,通过液体深层培养后,从中分离到8个新色原酮类次级代谢产物Sarcasciols A-H (1-8),应用质谱和核磁共振技术确定了它们的结构,用改进Mosher's方法确定了sarcasciol (1)中C-6位的绝对构型.并用96孔板法检测化合物的抑菌活性.