食用菌的原基形成受到环境因子和内源基因的共同调控,转录因子在其生长发育过程中能够起到关键作用.本研究在已测序金针菇单核菌株L11全基因组中鉴定到29个高速泳动蛋白(high-mobility-group box,HMG-box)转录因子编码基因.基于金针菇双核菌株H1123各发育时期转录组数据,筛选到一个与金针菇原基形成相关的HMG-box转录因子基因Fv-hmg.通过同源比对发现Fv-hmg的DNA序列在6个不同金针菇菌株中十分保守,一致性为98.38％,有92个SNP位点.克隆分析表明,该基因长度为1 517bp,含有一个内含子.该基因编码489个氨基酸残基的蛋白序列,含有一个HMG-box结构域.通过实时荧光定量PCR和转录组测序共同分析表明,该基因在金针菇原基时期的表达量比双核菌丝时期高达4倍以上,具有显著性差异(P＜0.01),由此推测该转录因子参与调控金针菇的原基形成.HMG-box转录因子Fv-Hmg在金针菇原基形成过程中的作用仍需进一步研究.%Primordium formation of edible mushroom is controlled by both environment factors and endogenous genes.Transcription factors (TFs) play important roles in the development process of mushroom.Twenty-nine high-mobility-group box (HMG-box) TF genes were identified from Flammulina velutipes based on whole genome analysis of the monokaryotic strain L11.Fv-hmg gene,one of the HMG-box TFs putatively involved in primordium formation,was analyzed using transcriptome of dikaryotic strain H1123.Homologous alignment indicated that the DNA sequence of Fv-hmg is highly conserved among six strains of F.velutipes (average identity=98.38％) with 92 SNP sites.The length of Fv-hmg gene is 1 517bp,which contains one intron.This gene can encode a protein with 489aa that contains a HMG-box domain.The expression level of Fv-hmg gene in different development stages was analyzed by both qRT-PCR and transcriptome methods.The expression level of this gene was four times higher in primordium than that in dikaryon stage,showing significant difference at P＜0.01 level.The results suggested that Fv-hmg gene might be involved in primordium formation of F.velutipes.The function of HMG-box TF Fv-Hmg in primordium formation of F.velutipes remains to be further investigated.
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