In order to cognize the effects of arbuscular mycorrhizal (AM) fungi on the growth,photosynthetic characteristics and the physiology of cut flowers,Tulipa gesneriana plant was inoculated with AM fungus Funneliformis mosseae,Claroideoglomus etunicatum,and F.mosseae+C.etunicatum (Fm+Ce) under greenhouse pot culturing conditions and uninoculated control was set up.The results showed that Fm+Ce inoculation treatment resulted in the increase of chlorophyll a,chlorophyll b and total chlorophyll,reaching 32％,18％ and 28％ respectively as compared with the uninoculated control.Compared with uninoculated control,the net photosynthetic rate,stomatal conductance,intercellular CO2 concentration and transpiration rate of leaves of tulip plants inoculated with AM fungi were significantly increased.Tulip plants inoculated with Fm+Ce reached the maximum photosynthetic parameters at 12 o'clock at noon.The scape length,shoot fresh weight,shoot dry weight,and leaf surface area were increased and the flowering period was earlier as compared with the control.AM fungal inoculation treatment could effectively increase the diameter of flowers,content of the soluble sugar and soluble protein,and the activity of superoxide dismutase and peroxidase during bottle inserting period,and decrease the content of malondialdehyde and relative conductivity in petals.AM fungi could effectively improve the water balance of cut flower,and prolong the lifespan,best viewing period,and florescence.%为认识丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对郁金香Tulipa gesneriana生长、光合特性以及切后瓶插期生理的影响,通过温室盆栽接种试验,以摩西斗管囊霉Funneliformis mosseae和幼套近明球囊霉Claroideoglomus etunicatum分别单独接种和共同接种,进行温室盆栽实验.结果表明,共同接种F.mosseae和C.etunicatum的郁金香叶片叶绿素a含量、叶绿素b含量和总叶绿素含量均显著高于不接种对照,分别增加了32％、18％和28％与不接种对照相比,接种AMF处理的郁金香叶片的净光合速率、气孔导度、胞间CO2浓度和蒸腾速率均显著提高,共同接种F.mosseae 和C.etunicatum的郁金香在正午12点达到光合参数最大值.接种AMF处理的郁金香花葶长、地上干物质质量、地上鲜物质质量和叶面积均高于不接种对照,开花期早于不接种对照.切花瓶插期间,接种AMF处理的郁金香切花花瓣可溶性糖含量、可溶性蛋白质含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)等抗氧化酶活性比不接种对照显著提高;且降低了膜脂过氧化产物丙二醛(MDA)含量和相对电导率.接种处理有效地改善切花花枝的水分平衡,并延长郁金香切花的瓶插寿命、最佳观赏期和花期.
展开▼
