蛇足石杉内生真菌无柄盘菌Pezicula sp.JR14中抑制乙酰胆碱酯酶的次级代谢产物分离

摘要

采用形态学及ITS-rDNA序列分析法,对具有抑制乙酰胆碱酯酶活性的蛇足石杉内生菌株JR14进行鉴定,确定为无柄盘菌Pezicula sp..开展了乙酰胆碱酯酶抑制活性化合物的分离,从Pezicula sp.JR14乙酸乙酯提取物中分离到4个化合物,利用核磁共振及质谱技术将其鉴定为behenic acid (1)、himeic acid B(2)、secalonic acid A (3)和palmitic acid (4).采用改进的Ellman比色法对分离的化合物进行活性检测,结果表明,himeic acid B(2)具有较强的乙酰胆碱酯酶抑制活性,其ICs0为205μg/mL (0.64mmol/L).%An endophytic fungus strain JR14 with acetylcholinesterase (ACHE) inhibitory activity from Huperzia serrata was identified as Pezicula sp.by morphological characteristics and internal transcribed spacer (ITS) sequence analysis.Four kinds of secondary metabolites were separated and purified from the ethyl acetate extract of the endophytic Pezicula sp.JR14.According to mass spectrometry,1H-NMR and 13C-NMR analyses,they were identified as behenic acid (1),himeic acid B (2),secalonic acid A (3) and palmitic acid (4).Ellman colorimetric test proved that himeic acid B (2) showed better AChE inhibitory activity with IC50 value of 205μg/mL (0.64mmol/L).