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Integration of Cytological Features with Molecular and Epigenetic Properties of Rice Chromosome 4

机译:水稻染色体4的细胞学特征与分子和表观遗传特性的整合

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摘要

It has been reported that rice chromosome 4 has eight major heterochromatic knobs within the heterochromatic half and that this organization correlates with chromosomal-leveI transcriptiOnaI activity.To better understand this chromosomaI organization,we created a modeI based on the statisticaI distribution of various types of gene models to divide chromosome 4 into 17 euchromatic and heterochromatic regions that correspond with the cytological staining.Fluorescence in.situ hvbridization(FISH)experiments using a set of bacteriaI artificiaI chromosome(BAC)clones from chromosome 4 placed all 18 clones in the region predicted by the model.Elevated levels of H3K4 di-and tri-methylation detected by chromatin-immunoprecipitation(CHIP)on chip were correlated with euchromatic regions whereas lower levels of these two modifications were detected in heterochromatic regions.Small RNAs were more abundant in the het erochromatic regions.To validate these findings,H3K4 trimethylation,H3K9 acetylation,H4K12 acetylation,and H3K9 di-and tri-methylation of 19 Individual genes were measured by Chlp-PCR.Genes in heterochromatic regions had elevated H3K9 di-and tri-methylation while genes in euchromatic regions had elevated levels of the other three modIfications.We also assayed cytosine methylation of these genes using the restriction enzymes McrBC,Hapll.and Msp I.This analysis indicated that cytosines of transposable elements and some genes located in heterochromatic regions were methylated while cytosines of the other genes were unmethylated.These results suggest that IocaI transcriptional activity may retiect the organization of the corresponding part of the chromosome.They also indicate that epigenetic regulation plays an important role in correlating chromosomal organization with transcriptionaI activity.
机译:据报道,水稻第4号染色体在异色一半内具有八个主要的异色结,并且该组织与染色体水平的转录活性相关。为了更好地了解这种染色体组织,我们基于各种基因的统计分布创建了一种modeI。该模型将4号染色体分为与细胞学染色相对应的17个常色和异色区域。使用来自4号染色体的一组细菌人工染色体(BAC)克隆进行荧光原位杂交(FISH)实验,将所有18个克隆置于芯片上的染色质免疫沉淀(CHIP)检测到的H3K4二甲基和三甲基化水平升高与常色区相关,而在异色区中检测到这两个修饰的水平较低。在异色区中小RNA含量较高。为了验证这些发现,H3K4三甲基化,H3K9乙酰化Chlp-PCR检测19个个体基因的H3K9乙酰化,H4K12乙酰化和H3K9二甲基化和三甲基化。异色区域中的基因的H3K9二甲基化和三甲基化水平升高,而常色区域中的基因的其他三个修饰水平升高。我们还使用限制酶McrBC,Hapll和Msp I分析了这些基因的胞嘧啶甲基化。此分析表明,转座因子的胞嘧啶和位于异色区域的某些基因被甲基化,而其他基因的胞嘧啶未被甲基化。 IocaI转录活性可能使染色体相应部分的组织消失。它们还表明表观遗传调控在使染色体组织与转录活性相关中起着重要作用。

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  • 来源
    《分子植物(英文版)》 |2008年第5期|816-829|共14页
  • 作者单位

    National Institute of Biological Sciences,8eijing 102206,China;

    National Institute of Biological Sciences,8eijing 102206,China;

    Peking-Yale Joint Research Center for Plant Molecular and Agrobiotechnology,College of Life Sciences,Peking University,Beijing 100871,China;

    Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;

    Department of Molecular,Cellular,and Developmental Biology,Yale University,New Haven,CT 06520-8104,USA;

    Department of Bioloqy,Wilkes University,WiIkes,Barre,PA 18766,USA;

    National Institute of Biological Sciences,8eijing 102206,China;

    Peking-Yale Joint Research Center for Plant Molecular and Agrobiotechnology,College of Life Sciences,Peking University,Beijing 100871,China;

    Department of Molecular,Cellular,and Developmental Biology,Yale University,New Haven,CT 06520-8104,USA;

    National Institute of Biological Sciences,8eijing 102206,China;

    National Institute of Biological Sciences,8eijing 102206,China;

    Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;

    National Institute of Biological Sciences,8eijing 102206,China;

    Department of Molecular,Cellular,and Developmental Biology,Yale University,New Haven,CT 06520-8104,USA;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 植物学;
  • 关键词

  • 入库时间 2022-08-19 03:37:53
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