目的：探讨芹菜素对人舌鳞癌Tca8113细胞的抗肿瘤作用与博来霉素的化疗增敏作用。方法不同浓度芹菜素（AP，20~160μmol/L）联合博来霉素50mg/L作为实验组，不同浓度芹菜素和博来霉素单独用药作为实验对照组，细胞正常培养为空白对照组。MTT法检测人舌鳞癌Tca8113细胞的增殖抑制作用，Annexin V-FITC/PI流式细胞术检测40μmol/L的AP联合50mg/L博来霉素作用于Tca8113细胞48h后细胞凋亡率。结果 MTT法检测增殖的抑制作用的强度随着AP作用浓度的增高、作用时间的延长而增强，实验组与空白对照组、实验对照组相比效果更明显（P＜0.05）；实验组凋亡率明显高于实验对照组，差异有统计学意义（P＜0.05）。结论芹菜素抑制舌鳞癌Tca8113细胞增殖，促进其凋亡，增强博来霉素对舌鳞癌Tca8113细胞的抗肿瘤作用。%Objective To explore the proliferation and apoptosis of combinative application of apigenin (AP) and bleomycin on Tca8113 cells. Methods Tca8113 cells were cultivated in vitro. Combinative application with different concen-tration of AP(20μmol/L, 40μmol/L, 80μmol/L, 160μmol/L) and bleomycin 50mg/L were cultivated as the experimental groups, single different concentration of AP and bleomycin was cultivated as control group , normal cells were cultivated as blank control group. All the groups were cultivated for 24,48,72 hours respectively. The anti-proliferative effect of Tca8113 cells was evaluat-ed by MTT assy. The apoptosis rate of AP (40μmol/L) combined with bleomycin (50μmol/L) was detected by flow cytometry with Annexin V-FITC/PI staining technique. Results The inhibition increased with concentration increasing and duration ex-tension,the experimental groups showed more obvious effects than control group and blank control group (P<0.05). The apoptosis rate on Tca8113 cells of experimental group was higher than control group (P<0.05). Conclusion AP can inhibit Tca8113 cells proliferation and induce cells apoptosis, enhance anti-tumor effect of bleomycin on Tca8113 cells.
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